Journal: Bioengineering

Article Title: Probing the Antitumor Mechanism of Solanum nigrum L. Aqueous Extract against Human Breast Cancer MCF7 Cells

doi: 10.3390/bioengineering6040112

Figure Lengend Snippet: Percentage increase (mean ± standard deviation) in cytotoxicity of AESN (concentration: 5 g/L and 10 g/L) against human mammary gland epithelial cell line MCF12A and breast cancer cell line MCF7. The cytotoxicity was measured 72 h of treatment using the CytoTox 96 Non-radioactive Cytotoxicity Assay. Cells treated with water were used as a negative control.

Article Snippet: Human mammary gland epithelial cell line MCF12A and breast cancer luminal A subtype cell line MCF7 were purchased from the American Type Culture Collection (Manassas, VA, USA).

Techniques: Standard Deviation, Concentration Assay, Cytotoxicity Assay, Negative Control

Journal: Bioengineering

Article Title: Probing the Antitumor Mechanism of Solanum nigrum L. Aqueous Extract against Human Breast Cancer MCF7 Cells

doi: 10.3390/bioengineering6040112

Figure Lengend Snippet: Determination of the inhibitory effect of ASEN (concentration: 10 g/L) on human breast cancer MCF7 cell migration using the wound healing migration assay.

Article Snippet: Human mammary gland epithelial cell line MCF12A and breast cancer luminal A subtype cell line MCF7 were purchased from the American Type Culture Collection (Manassas, VA, USA).

Techniques: Concentration Assay, Migration

Journal: Bioengineering

Article Title: Probing the Antitumor Mechanism of Solanum nigrum L. Aqueous Extract against Human Breast Cancer MCF7 Cells

doi: 10.3390/bioengineering6040112

Figure Lengend Snippet: Determination of the effects of ASEN (10 g/L) on hexokinase activity ( A ), pyruvate kinase activity ( B ) and mitochondrial membrane potential ( C ) in human breast cancer MCF7 cells using the Hexokinase Activity Assay Kit, Pyruvate Kinase Activity Assay Kit and JC-1-Mitochondrial Membrane Potential Assay Kit, respectively. Treatment with water was used as a negative control.

Article Snippet: Human mammary gland epithelial cell line MCF12A and breast cancer luminal A subtype cell line MCF7 were purchased from the American Type Culture Collection (Manassas, VA, USA).

Techniques: Activity Assay, Membrane, Kinase Assay, Negative Control

Journal: Bioengineering

Article Title: Probing the Antitumor Mechanism of Solanum nigrum L. Aqueous Extract against Human Breast Cancer MCF7 Cells

doi: 10.3390/bioengineering6040112

Figure Lengend Snippet: Venn diagram representations of ( A ) genes differentially expressed upon AESN treatment in MCF7 cells and disease genes and ( B ) genes differentially expressed upon AESN treatment in MCF7 cells and druggable genes.

Article Snippet: Human mammary gland epithelial cell line MCF12A and breast cancer luminal A subtype cell line MCF7 were purchased from the American Type Culture Collection (Manassas, VA, USA).

Techniques:

Journal: Bioengineering

Article Title: Probing the Antitumor Mechanism of Solanum nigrum L. Aqueous Extract against Human Breast Cancer MCF7 Cells

doi: 10.3390/bioengineering6040112

Figure Lengend Snippet: Bar chart representation of genes that are differentially expressed upon AESN treatment in breast cancer MCF7 cells and their involvement in various diseases.

Article Snippet: Human mammary gland epithelial cell line MCF12A and breast cancer luminal A subtype cell line MCF7 were purchased from the American Type Culture Collection (Manassas, VA, USA).

Techniques: